Which clotting factors are most affected by temperature?

The correct answer is based on the fact that Factors V and VIII are particularly sensitive to changes in temperature due to their structure and function. Factor V is a labile factor that is quickly inactivated at elevated temperatures and is essential in the cascade of events that leads to the formation of a fibrin clot. Similar to Factor V, Factor VIII also has a high turnover rate and can be affected by thermal conditions because it is a large glycoprotein and is sensitive to proteolysis and denaturation, especially outside the optimal conditions for enzyme activity.

This sensitivity means that in conditions of hypothermia or abnormal storage, the levels and functional activity of Factors V and VIII may decline, impacting the overall hemostatic process. In contrast, other factors listed in the options do not exhibit the same level of temperature sensitivity, as they are either more stable or less affected by environmental changes.